Compound | R | R | Ki (mM) |
---|---|---|---|
Heroin | —COCH3 | —COCH3 | 0.3 |
6-Acetylmorphine | H | —COCH3 | 0.3 |
Morphine | H | H | >10 |
Ki values of heroin, 6-monoacetylmorphine, and morphine were determined by their competitive inhibition of the hydrolysis of 4-methylumbelliferylacetate with purified hCE-1 as described in Methods.