Table 3

Effect of antibodies on 7EFC O-deethylase and reference activities in human liver microsomes

Human Liver Sample	Antibody	7EFC O-Deethylase Activity	Reference Assay	Reference Assay Activity
		%		%
HLS2	MAB-2A6	80	Coumarin 7-hydroxylase	18
HLS9	MAB-2A6	108	Coumarin 7-hydroxylase	14
HLS5	MAB-2E1	88	p-Nitrophenol hydroxylase	10
HLS6	MAB-2E1	67	p-Nitrophenol hydroxylase	10
HLS5	Anti-CYP1A2	56	PhenacetinO-deethylase	8
HLS9	Anti-CYP1A2	100	PhenacetinO-deethylase	8
HLS5	Anti-CYP2C13	89	Diclofenac 4′-hydroxylase	15
HLS5	Anti-CYP2C13	89	(S)-Mephenytoin 4′-hydroxylase	22

7EFC O-deethylase (5 μM substrate), phenacetinO-deethylase (200 μM substrate), diclofenac 4′-hydroxylase (100 μM substrate), (S)-mephenytoin 4′-hydroxylase (100 μM substrate), p-nitrophenol hydroxylase (50 μM substrate), and coumarin 7-hydroxylase (200 μM substrate) activities were assayed as described in Materials and Methods.Antibodies were used as described in Materials and Methods.Values shown are the means of two determinations and represent the percentage of activity in the absence of anti-CYP antibodies.