Rate constants for inactivation of purified 2B1 enzymes2-a
| Enzyme | Chloramphenicol | N-(2-p-nitrophenethyl) Chlorofluoroacetamide |
|---|---|---|
| 50 μM | 250 μM | |
| 2B1 E322V | 0.21 (67)2-b | 0.27 (44) |
| 2B1 E322V/V103A/E424K | 0.23 (63) | 0.24 (53) |
↵2-a The ki values (min−1) represent the average of two independent experiments with the indicated inhibitor concentration. Experimental conditions and details are described in “Materials and Methods.” Control values (methanol) were all ≤0.01 min−1.
↵2-b Numbers in parentheses represent the percent activity remaining at the zero time point, as determined by linear regression analysis.