Effect of once-daily and twice-daily sc injections of unesterified testosterone during puberty on CYP2C11 enzyme activity and protein content in hepatic microsomes isolated from prepubertally ovariectomized adult rats1-a
| Treatment | N | Testosterone 2α-Hydroxylase Activity1-b | CYP2C11 Protein Content1-c |
|---|---|---|---|
| Corn oil (vehicle control) | 7 | <0.031-d | <0.011-e |
| Unesterified testosterone (5 μmol/kg once daily) | 7 | <0.03 | <0.01 |
| Unesterified testosterone (2.5 μmol/kg twice daily) | 8 | 0.04 ± 0.03 | 0.010 ± 0.005 |
↵1-a Prepubertally (day 25 of age) ovariectomized rats were injected subcutaneously with unesterified testosterone (5 μmol/kg once daily or 2.5 μmol/kg twice daily) or corn oil (1 ml/kg, vehicle control) at days 35–49 of age (puberty) and sacrificed at day 90 of age (adult). Results are expressed as mean ± SE for the number (N) of rats indicated per treatment group.
↵1-b Activity expressed as nmol/min/nmol total microsomal P450.
↵1-c Protein content expressed as nmol/nmol total microsomal P450.
↵1-d Below the limit of quantitation of 0.03 nmol/min/nmol total microsomal P450.
↵1-e Below the limit of quantitation of 0.01 nmol/nmol total microsomal P450.