Table 8

Metabolites generated in dexamethasone-induced rat liver microsomes incubated with L-732,531

Peak	[M + H⁺]⁺	Fragment Ions	Mass Difference From Parent	Proposed Biotransformation
	m/z	m/z
1	923	905, 887, 536	−28	Di-demethylated at 13 and 15 positions
2	951	933, 915, 564	0	Tautomer of L-732,531
3	923	905, 887, 536	−28	Didemethylated at 13 and 15 positions
4	937	919, 901, 550	−14	Demethylated at 13 or 15 position
5	937	919, 901, 550	−14	Demethylated at 13 or 15 position
6	967	949, 931, 580	+16	Addition of a hydroxyl group (on C1-25)
7	965	947, 578	+14	Addition of oxygen with subsequent oxidation (on C1-25)
8	937	919, 901, 550	−14	Demethylated at 13 or 15 position
9	965	947, 929, 578	+14	Addition of oxygen with subsequent oxidation (on C1-25)
10	965	947, 929, 578	+14	Addition of oxygen with subsequent oxidation (on C1-25)
11	949	931, 913, 562	−2	Desaturated or hydroxylated w/loss of H₂O (on C1-25)
12	937	919, 901, 550	−14	Demethylated at 13 or 15 position
13	951	933, 915, 564	0	Tautomer of L-732,531
14	951	933, 915, 564	0	L-732,531

The peaks are labeled 1–14 according to their HPL retention times, as shown in fig. 6.