Effects of cofactors on CCMF and CPPA generated from MCF by human liver S9 fractions
| Compound | Analysis | Metabolite Formation | ||
|---|---|---|---|---|
| NADP+ | NAD+ | NADP+ + NAD+ | ||
| % | ||||
| CCMF | GC/MS (SIM) | 100 ± 6 | 45 ± 5 | 63 ± 6 |
| CCMF | HPLC | 100 ± 10 | 52 ± 14 | 75 ± 4 |
| CPPA | HPLC | 5 ± 1 | 100 ± 19 | 20 ± 1 |
Values represent peak areas that were normalized to the corresponding highest values and are expressed as mean ± SD (N = 3). Incubations were carried out in the presence of an NADPH-generating system, as described in Materials and Methods. Separate incubation samples were prepared for the HPLC and GC/MS analyses, which were performed as described inMaterials and Methods.