Summary of Tri metabolism to DCVG in kidney and liver cells or subcellular fractions from F344 rats and B6C3F1 mice
| DCVG Formation (nmol/mg protein or 106 cells per 60 min) | ||
|---|---|---|
| Male | Female | |
| Rat kidney cells | 0.48 ± 0.023-a | 0.65 ± 0.15 |
| Rat hepatocytes | 9.70 ± 0.29a,b | 2.67 ± 0.69 |
| Rat kidney cytosol | 0.45 ± 0.22c,d | 0.32 ± 0.023-d |
| Rat kidney microsomes | NDb,c | 0.61 ± 0.06 |
| Rat liver cytosol | 7.30 ± 2.80a,c | 4.86 ± 0.143-d |
| Rat liver microsomes | 10.3 ± 2.8a,c | 7.24 ± 0.24 |
| Mouse kidney cytosol | 5.60 ± 0.243-b | 3.70 ± 0.483-d |
| Mouse kidney microsomes | 5.47 ± 1.413-b | 16.7 ± 4.7 |
| Mouse liver cytosol | 24.5 ± 2.43-d | 21.7 ± 0.9 |
| Mouse liver microsomes | 40.0 ± 3.13-b | 25.6 ± 0.8 |
Results are means ± SE of measurements from three separate cell or tissue fractionation preparations and are for 2 mM Tri incubated with 5 mM GSH for 60 min. DCVG formation was measured after derivatization of acid extracts with iodoacetate and 1-fluoro-2,4-dinitrobenzene, separation by ion exchange, gradient HPLC on an amine column using a methanol-acetate mobile phase, and detection of N-dinitrophenyl DCVG at 365 nm.
↵3-a Data derived from ref. 4.
↵3-b Significantly different (p < 0.05) from DCVG formation in same species and tissue sample in females.
3-c Significantly different (p < 0.05) from DCVG formation in corresponding sample in mice of same sex.
↵3-d Significantly different (p < 0.05) from DCVG formation in microsomes from same sex, species, and tissue.