Experiment1-a | NDMA Demethylase Activity1-b |
---|---|
nmol [14C]HCHO/mg protein/min | |
−NADPH + EC | 0.95 ± 0.03 |
−PAX − EC | 0.97 ± 0.03 |
+PAX − EC | 0.96 ± 0.01 |
−PAX + EC | 0.77 ± 0.011-c |
+PAX + EC | 0.39 ± 0.01c,d |
−NADPH + EC | 0.98 ± 0.03 |
−PMSF − EC | 0.99 ± 0.03 |
+PMSF − EC | 1.09 ± 0.10 |
−PMSF + EC | 0.78 ± 0.011-e |
+PMSF + EC | 0.32 ± 0.04e,f |
↵1-a Incubations were conducted in the presence of an NADPH-generating system unless otherwise indicated.
↵1-b Activity of NDMA demethylase was determined by measuring the formation of [14C]HCHO, as described in Materials and Methods. Data are expressed as mean ± SD of quadruplicate determinations from three or four different microsomal preparations. Pairwise multiple comparisons of experimental groups were performed by the Student-Newman-Keuls test (p < 0.05).
↵1-c Significantly different from −NADPH + EC, −PAX − EC, and +PAX − EC.
1-d Significantly different from −PAX + EC.
↵1-e Significantly different from −NADPH + EC, −PMSF − EC, and +PMSF − EC.
1-f Significantly different from −PMSF + EC.