Effects of immunoinhibition with an anti-CYP2E1 Mab on levels of covalent binding of [ethyl-14C]EC to liver microsomal proteins
| Experiment4-a | Covalent Binding4-b |
|---|---|
| pmol [ethyl-14C]EC/mg protein | |
| −Mab + EC − NADPH | 3.09 ± 0.26 |
| −Mab + EC + NADPH | 12.34 ± 1.564-c |
| +Mab + EC + NADPH | 4.42 ± 0.42 |
| +HyHel 9 + EC + NADPH | 13.61 ± 1.384-c |
↵4-a Liver microsomes were incubated with an anti-CYP2E1 inhibitory antibody (Mab 1-91-3) or a nonspecific antibody (Mab HyHel 9) at an antibody protein/microsomal protein ratio of 0.5.
↵4-b Data are expressed as mean ± SD of quadruplicate determinations from three or four different microsomal preparations. Pairwise multiple comparisons of experimental groups were performed by the Student-Newman-Keuls test (p < 0.05).
↵4-c Significantly different from −Mab + EC − NADPH and +Mab + EC + NADPH.