Relative mRNA Levels | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Untreated | TH | ADS | OZ | CL | ||||||
−ACF | +ACF | −ACF | +ACF | −ACF | +ACF | −ACF | +ACF | −ACF | +ACF | |
mEH | 1 | 0.4 ± 0.2 | 17.9 ± 4.9 | 9.1 ± 2.51-a | 9.1 ± 3.8 | 0.3 ± 0.21-a | 14.4 ± 3.5 | 1.1 ± 0.21-a | 7.0 ± 1.6 | 2.8 ± 0.71-a |
rGSTA2 | 1 | 0.8 ± 0.5 | 14.3 ± 6.8 | 10.2 ± 3.2 | 12.0 ± 5.6 | 1.0 ± 0.61-a | 18.4 ± 7.1 | 2.3 ± 0.21-a | 13.7 ± 6.0 | 5.6 ± 2.01-a |
rGSTA3 | 1 | 0.7 ± 0.4 | 2.3 ± 0.3 | 1.3 ± 0.11-a | 2.4 ± 0.5 | 1.6 ± 0.21-a | 2.7 ± 0.4 | 1.0 ± 0.11-a | 1.4 ± 0.1 | 1.3 ± 0.2 |
rGSTM1 | 1 | 0.5 ± 0.21-a | 2.6 ± 0.1 | 1.7 ± 0.11-a | 1.8 ± 0.5 | 0.4 ± 0.21-a | 1.9 ± 0.1 | 0.8 ± 0.11-a | 1.7 ± 0.1 | 0.8 ± 0.11-a |
rGSTM2 | 1 | 0.6 ± 0.21-a | 1.9 ± 0.1 | 1.6 ± 0.1 | 1.3 ± 0.4 | 0.4 ± 0.21-a | 1.8 ± 0.1 | 0.5 ± 0.11-a | 2.2 ± 0.1 | 0.5 ± 0.11-a |
Northern blot analysis was performed with total RNA fractions isolated from untreated animals or rats at 24 hr after treatment with a single dose of TH (100 mg/kg, ip), ADS (100 mg/kg, po), OZ (100 mg/kg, po), or CL (100 mg/kg, po), with or without a concomitant injection of ACF (20 mg/kg, im). Changes in the mRNA levels, relative to GAPDH, were assessed by scanning densitometry. Each value represents the mean ± SD from three separate animal experiments. Data were analyzed by one-way analysis of variance followed by the Newmann-Keuls test for comparison of multiple group means.
↵1-a p < 0.05, in comparison with the respective −ACF control.