Table 2

Activity of azonafide and metabolites in MTT assays2-a with CHO cells

AgentIC50 (nM) [SD]
Azonafide68.2 [5]
Mono-N′-desmethyl metabolite265 [20]
Di-N′-desmethyl metabolite981 [27]
N′-Oxide metaboliteNA2-b
Carboxylic acid metaboliteNA

  • ↵2-a Continuous drug exposure time was 1 hr in the MTT assays; total incubation time in assays was 5 days. Results are based on three independent experiments for each agent.

  • ↵2-b Not active.