Table 3

Michaelis-Menten parameters obtained for hydroxycoumarin formation in hepatic microsomes and isolated hepatocytes

VmaxKMCL1CL2CLHC
pmol/min μM μl/min μl/min μl/min
Microsomes3-a 39.8  ± 9.80.9  ± 0.147.7  ± 17.42.1  ± 1.049.8  ± 18.6
Hepatocytes3-b 14.1  ± 6.10.8  ± 0.524.3  ± 2.12.1  ± 0.726.4  ± 16.8

  • Data are presented as mean ± SD (N = 4).

  • ↵3-a Vmax and clearance terms are expressed per milligram of microsomal protein.

  • ↵3-b Vmax and clearance terms are expressed per 106 cells.