Catalyst | Concentration | Specific Activity | |
---|---|---|---|
13-cRA as Substrate | 9-cRA as Substrate | ||
pmol/mg3-a | |||
RCH | 0.35 mg protein/ml | 742 ± 34 | 552 ± 27 |
GSH | 2.5 mM | 369 ± 37 | 268 ± 33 |
GSH | 5.0 mM | 67 ± 11 | 87 ± 23 |
GSH | 7.5 mM | 45 ± 8 | 58 ± 7 |
GSH | 10.0 mM | 27 ± 4 | 31 ± 7 |
Incubations of 13-cRA or 9-cRA (3.3 μM) were with RCH or GSH in 0.1 M sodium phosphate buffer (pH 7.5) and were conducted at 37°C for 5 min in the dark. Final volumes of the reaction mixtures were 1 ml.
↵3-a Picomoles per milligram of protein or GSH.