Purification of FMO3 (A) and FMO1 (B) from sheep liver microsomes
| Fractions | Total Proteins (mg) | Total Activity (nmol/min) | Specific Activity (*) (nmol/min/mg) | Purification Factor | Rendement % |
|---|---|---|---|---|---|
| A. FMO3 | |||||
| Microsomes | 981 | 4218 | 4.3 | 1 | 100 |
| Solubilized microsomes | 396 | 2219 | 5.6 | 1.3 | 53 |
| Pool DE 52 | 197 | 1779 | 9 | 2.1 | 42 |
| Pool blue Sepharose | 75.2 | 1293 | 17.2 | 4 | 31 |
| Pool hydroxyapatite | 57 | 1201 | 21.1 | 4.9 | 28 |
| 2′,5-ADP | 0.29 | 298 | 1025 | 240 | 7.1 |
| Fractions | Total Proteins (mg) | Total Activity (nmol/min) | Specific Activity (*) (nmol/min/mg) | Immunoreactivity (**) | |
|---|---|---|---|---|---|
| Anti FMO1 | Anti FMO3 | ||||
| B. FMO1 | |||||
| Solubilized microsomes | 1177 | 5354 | 4.5 | + | + |
| Blue Sepharose | |||||
| Unbound fraction | 1092 | 5120 | 4.7 | − | + |
| Bound fraction | 38.5 | 212 | 5.5 | + | − |
| Purified fraction preparative electrophoresis | 0.65 | ND | ND | + | − |
| Partially purified fraction hydroxyapatite | 2.8 | 12.6 | 4.5 | + | − |