Purification of the NM441-hydrolase from rat serum (36 ml)
| Step | Total Protein | Total Activity | Specific Activity | Purification | Yield |
|---|---|---|---|---|---|
| mg | nmol/min | nmol/min/mg | fold | % | |
| Serum | 1863 | 1856 | 1.00 | 1 | 100 |
| HDL fraction | 30.6 | 2103 | 68.7 | 69 | 113 |
| Heparin-Sepharose | 18.9 | 1627 | 86.1 | 86 | 88 |
| Solubilization | 18.5 | 1453 | 78.5 | 79 | 78 |
| Mono Q | 0.46 | 317 | 689 | 689 | 17 |