Substrate | Nat1 | Nat2 | Nat3 |
---|---|---|---|
Isoniazid1-a | 35.04 ± 1.421-b | ND | ND |
Sulfamethazine | 0.86 ± 0.06 | 0.08 ± 0.01 | ND |
PABA | 0.44 ± 0.22 | 9.14 ± 0.31 | ND |
pABG | ND | 15.30 ± 0.80 | ND |
2-AF | 2.03 ± 0.19 | 10.88 ± 1.17 | ND |
5-ASA | 7.19 ± 0.49 | 17.56 ± 0.39 | 0.85 ± 0.08 |
ND, less than 0.04 nmol/min/mg of protein for sulfamethazine,p-aminobenzoylglutamate (PABA), pABG, 2-aminofluorene (2-AF), and 5-aminosalicyclic acid (5-ASA); less than 0.1 nmol/min/mg of protein for INH.
↵1-a Assay differed substantially from all other substrates (see text).
↵1-b Values presented are averages of specific activity (nmol/min/mg total protein) ± standard errors (N = 3). Background values for activity (<0.04 nmol/min/mg of protein) have been subtracted.