Table 1

Summary of key MS fragmentation for tolterodine and its metabolites in dog and mouse urine after electrospray ionization and collision-induced dissociation

Metabolite[M+H]+CID1-aFragments
Tolterodine328286, 199, 182, 149, 123, 91
5-HM344302, 225, 215, 197, 154, 133, 105, 91
5-CM358316, 239, 229, 211, 194, 181, 165, 153, 133, 105, 91
N-Dealkylated tolterodine286199, 181, 149, 123, 91
N-Dealkylated 5-HM300223, 213, 195, 177, 165, 167, 152, 133, 105, 91
N-Dealkylated 5-CM316239, 229, 211, 181, 165, 153, 133, 105, 91
Tolterodine glucuronide504328, 286, 199, 182, 149
5-HM glucuronide518342, 233, 213, 195
5-CM glucuronide534358, 316, 239, 229, 211, 179, 153
N-Dealkylated tolterodine glucuronide460284, 196, 147, 119
N-Dealkylated 5-HM glucuronide476213, 167
N-Dealkylated 5-CM glucuronide490

  • ↵1-a CID, collision-induced dissociation.