Recovery of testosterone 6β-hydroxylase activity (CYP3A4) after an 18-hr dialysis following a 20-min preincubation with nelfinavir mesylate and NADPH in human liver microsomes
| Nelfinavir | % of Control Activity Remaining | |
|---|---|---|
| Dialyzed (n = 3) Mean ± SD | Undialyzed (n= 2) Average (range) | |
| μM | ||
| 0 | 100 ± 2.3 | 100 (6.8) |
| 1.0 | 103 ± 2.3 | 88 (0.8) |
| 3.0 | 82 ± 1.6 | 70 (3.6) |
| 5.0 | 83 ± 0.8 | 51 (0.6) |
| 10.0 | 44 ± 15.5 | 26 (2.2) |
Values for dialyzed samples were calculated from three separate experiments, and for undialyzed samples the values were calculated from two separate experiments.