Incubation Mixture | Relative Activity3-a | |
---|---|---|
R = CH3 | R = CH (CH3)2 | |
% | ||
Complete | 100 | 100 |
−2-Oxo acid | ND3-b | ND |
−Mg2+ (2 mM) | 86 | 86 |
−TPP (0.2 mM) | 72 | 74 |
−NOB (2 mM) | ND | ND |
−Mitochondria | ND | ND |
Heated mitochondria | ND | ND |
↵3-a Activities in the formation ofN-hydroxy-N-phenylacetamide (R = CH3) and N-hydroxy-N-phenylisobutyramide [R = CH (CH3)2] are expressed relative to that of the complete mixture, arbitrarily taken as 100%. Heated mitochondria were prepared by heating for 20 sec in boiling water saturated with ammonium sulfate.
↵3-b ND, not determined.