Table 4

Kinetic constants for NOB in the mitochondrion-catalyzed formation ofN-hydroxy-N-phenylacylamides

2-Oxo Acid	R	S_0.5	V_max	V_max/S_0.5
		mM	nmol/min/mg	nmol/mM/min/mg
Glyoxylate	H			0.132 ± 0.002^4-a
Pyruvate	CH₃	0.332 ± 0.038	1.51 ± 0.07	4.55
2-Oxobutyrate	CH₂CH₃	0.394 ± 0.014	2.12 ± 0.03	5.38
2-Oxovalerate	CH₂CH₂CH₃	0.287 ± 0.024	0.704 ± 0.028	2.45
2-Oxoisovalerate	CH (CH₃)₂	0.351 ± 0.011	1.43 ± 0.06	4.07
2-Oxoisocaproate	CH₂CH (CH₃)₂	0.310 ± 0.060	0.775 ± 0.050	2.50
2-Oxo-3-methylvalerate	CH (CH₃) CH₂CH₃	0.380 ± 0.011	0.864 ± 0.070	2.27

↵4-a Second order rate constant (M⁻¹sec⁻¹) for the nonenzymatic formation ofN-hydroxy-N-phenylformamide at 37°C and pH 6.9. The reported value (Corbett and Corbett, 1980) measured at 30°C and pH 6.0 was 0.119 ± 0.010 M⁻¹ sec⁻¹.