Residue profiles of excreta, egg yolk, and tissues
| Specimen | Isotope | Residue Amount | |||||
|---|---|---|---|---|---|---|---|
| MAB1a | AB1a | 24-OH-MAB1a | 24-OH-AB1a | 24-OH-MAB1a Conjugates4-a | 24-OH-AB1a Conjugates4-b | ||
| % | |||||||
| Excreta4-c | 3H | 51.5 ± 1.5 | 5.2 ± 1.5 | 33.4 ± 1.5 | 1.0 ± 0.7 | ND4-d | ND |
| 14C | 51.9 ± 1.8 | 5.1 ± 1.5 | 32.7 ± 1.3 | 1.0 ± 0.7 | |||
| Egg yolk4-e | 3H | 26.1 | 1.7 | 4.4 | ND | 60.4 | 3.6 |
| 14C | 27.3 | 1.4 | 4.6 | 59.2 | 3.4 | ||
| Liver | 3H | 45.9 | 3.7 | 7.8 | ND | 31.0 | 5.2 |
| 14C | 49.3 | 3.9 | 7.2 | 27.8 | 5.1 | ||
| Liver4-f | 3H | 9.2 | 1.5 | 6.3 | ND | 70.9 | 7.4 |
| 14C | 11.6 | 2.0 | 6.3 | 67.9 | 6.9 | ||
| Liver4-g | 3H | 64.7 | 2.5 | 4.7 | ND | 24.0 | 1.7 |
| 14C | 68.9 | 3.2 | 4.2 | 19.8 | 1.5 | ||
| Thigh muscle | 3H | 67.3 | 2.7 | 4.0 | ND | 17.9 | 4.0 |
| 14C | 56.5 | 3.3 | 5.5 | 17.9 | 5.5 | ||
| Breast muscle | 3H | 81.0 | 2.9 | 4.6 | ND | 6.7 | 1.1 |
| 14C | 77.7 | 3.9 | 5.3 | 6.0 | 1.8 | ||
| Abdominal fat | 3H | 63.9 | 2.0 | 1.4 | ND | 28.7 | 1.7 |
| 14C | 64.4 | 2.0 | 1.5 | 28.1 | 1.4 | ||
| Muscle fat/skin | 3H | 67.0 | 2.0 | 1.6 | ND | 25.2 | 1.6 |
| 14C | 66.1 | 2.1 | 1.5 | 25.2 | 1.7 | ||
| Ovaries | 3H | 26.8 | 1.6 | 4.0 | ND | 58.6 | 4.2 |
| 14C | 26.9 | 1.7 | 4.2 | 57.7 | 4.0 | ||
HPLC method 1 (table 1) was used for all analyses. Residue amounts are expressed as percentages of total radioactivity recovered from the HPLC column. Recoveries were generally between 95 and 105%.
↵4-a A group of eight fatty acid conjugates of 24-OH-MAB1a that eluted as a single peak.
↵4-b A group of eight fatty acid conjugates of 24-OH-AB1a that eluted as a single peak.
↵4-c Average ± SD from four excreta samples (1, 3, 6, and 7 days).
↵4-d ND, not detected.
↵4-e Values from a composite of all egg yolk specimens.
↵4-f Liver with the highest TRR level.
↵4-g Liver with the lowest TRR level.