Specificity of the various CYPs with respect to the reactions shown to be specific for particular CYPs
| Reaction (substrate and enzyme conc.) | Activity of Each Isoform | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1A1 | 1A2 | 2C8 | 2C9 | 2C19 | 2D6 | 2E1 | 3A4 | 3A5 | |
| nmol/min/nmol CYP | |||||||||
| 7-EthoxyresorufinO-deethylation | 1.56 | 0.4 | |||||||
| (2.5 μM, 10 pmols) | |||||||||
| 6α-Paclitaxel hydroxylation | 0.96 | ||||||||
| (10 μM, 25 pmols) | |||||||||
| Diclofenac 4-hydroxylation | 14 | 1.7 | |||||||
| (100 μM, 10 pmols) | |||||||||
| S-Mephenytoin 4-hydroxylation | 0.8 | ||||||||
| (100 μM, 50 pmols) | |||||||||
| Chlorzoxazone 6-hydroxylation | 1.8 | 0.4 | 0.03 | 0.12 | 0.156 | 2.6 | 0.27 | ||
| (1 mM, 50 pmols) | |||||||||
| Bufuralol 1′-hydroxylation | 7.0 | ||||||||
| (50 μM, 3 pmols) | |||||||||
| Testosterone 6β-hydroxylation | 0.1 | 0.04 | 5.2 | 0.6 | |||||
| (100 μM, 30 pmols) | |||||||||