Glucuronidation of phenols and coumarins by stably expressed human UGT1A8 and 1A10
| Substrates | Glucuronide Formation | |
|---|---|---|
| UGT1A8 | UGT1A10 | |
| pmol/min/mg protein | ||
| Phenolic compounds | ||
| p-Nitrophenol | 287 | ND |
| Eugenol | 1322 | 112 |
| 1-Naphthol | 1410 | ND |
| Propofol | 1016 | ND |
| Carvacrol | 744 | ND |
| 4-Hydroxybiphenyl | 1200 | 19 |
| 2-Hydroxybiphenyl | 290 | ND |
| Mycophenolic acid | 1990 | 93 |
| Coumarins | ||
| Scopoletin | 11800 | 390 |
| 4-Hydroxycoumarin | 230 | ND |
| Umbelliferone | 665 | ND |
| 4-Methylumbelliferone | 1180 | 26 |
| Esculetin | 612 | ND |
Assays were performed using pH 7.4 Tris buffer at 37°C for 1.0 h and membrane preparations of HK293 cells expressing UGT1A8 or UGT1A10. The concentration of UDP-glucuronic acid was 2.0 mM and the aglycone concentrations were 0.5 mM. Values represent the average enzymatic rates from two different experiments.
ND, not detected (limit of detection is 2.0 pmol/min/mg protein).