In vitro enzyme kinetic parameters for the (S)-warfarin 7-hydroxylation and inhibition constant of bucolome for human liver and the recombinant CYP2C9 microsomes
| Kinetic Parameters | Human Liver Microsomes | CYP2C9 Microsomes |
|---|---|---|
| V max (pmol/mg protein/min) | 4.33 ± 0.15 | 18.9 ± 0.573-a |
| K m (μM) | 1.50 ± 0.23 | 1.86 ± 0.23 |
| K i (μM) | 20.2 ± 3.62 | 8.24 ± 1.48 |
| α | 8696 ± 349 | 1293 ± 527 |
Data are means ± S.D., which are asymptotic S.D.
↵3-a Equivalent to 282 pmol/nmol P-450/min.