P-450 Enzymes | Km | Vmax | Vmax/Km |
---|---|---|---|
μM | min−1 | ml/min/nmol | |
CYP2C8 | 2.7 ± 2.5 | 4.2 ± 1.0 | 1.6 |
CYP2C9 | 3.6 ± 2.9 | 0.6 ± 0.1 | 0.2 |
CYP2C19 | 3.1 ± 1.8 | 2.8 ± 0.3 | 0.9 |
CYP3A4 | 120 ± 5 | 47 ± 1 | 0.4 |
Kinetic analysis for metabolite 3 formation of troglitazone were determined with P-450 concentration of 0.025 μM (Supersomes; Gentest) and substrate concentration between 1 and 100 μM. The kinetic parameters were calculated from the fitted curves with the computer program KaleidaGraph.