Addition | μM | Metabolite 3 Formation | |||
---|---|---|---|---|---|
10 μM troglitazone | 30 μM troglitazone | ||||
HL-3 | HL-4 | HL-3 | HL-4 | ||
pmol/min/nmol P-450 | |||||
None | 191 ± 18 (100) | 138 ± 18 (100) | 292 (100) | 200 (100) | |
Quercetin | 10 | 204 ± 14 (107) | 80 ± 10∗ (58) | 387 (133) | 209 (97) |
Sulfaphenazole | 10 | 228 ± 16 (119) | 124 ± 9 (90) | 428 (147) | 200 (100) |
Fluvoxamine | 10 | 236 ± 21 (124) | 137 ± 11 (99) | 443 (152) | 212 (106) |
Quinidine | 10 | 211 ± 10 (110) | 141 ± 13 (102) | 319 (109) | 216 (108) |
Ketoconazole | 1 | 130 ± 11∗ (68) | 76 ± 6∗ (55) | 157 (54) | 100 (50) |
Combination of | |||||
quercetin and | 10 | 92 ± 14∗∗ (48) | 44 ± 16∗∗ (32) | n.d. | n.d. |
ketoconazole | 1 |
Troglitazone (10 or 30 μM) was incubated with human liver microsomes (samples HL-3 and HL-4) in the absence or presence of chemical inhibitors. Data are means ± S.D. of triplicate (10 μM troglitazone) or means of duplicate (30 μM) determinations.
Values in parenthesis indicate percentage of control (none). n.d., not done.
∗p < .05, ∗∗p < .01.