CYP2D | 8-Hydroxylation | N-Demethylation | ||||
---|---|---|---|---|---|---|
Km | Vmax | Vmax/Km | Km | Vmax | Vmax/Km | |
μM | nmol/min/nmol P-450 | ml/min/nmol P-450 | μM | nmol/min/nmol P-450 | ml/min/nmol P-450 | |
2D1 | 35.5 ± 4.9 | 1.83 ± 0.14 | 0.052 ± 0.005 | 156.5 ± 14.9 | 4.39 ± 0.21 | 0.028 ± 0.002 |
2D2 | 5.9 ± 0.7 | 2.26 ± 0.13 | 0.385 ± 0.054 | N.C. | N.C. | N.C. |
2D3 | 69.2 ± 6.8 | 2.73 ± 0.23 | 0.040 ± 0.007 | 84.5 ± 8.0 | 14.92 ± 2.18 | 0.176 ± 0.012 |
2D4 | 133.3 ± 12.6 | 9.26 ± 1.01 | 0.069 ± 0.006 | 402.7 ± 40.7 | 65.13 ± 4.98 | 0.162 ± 0.015 |
2D6 | 3.7 ± 0.3 | 2.90 ± 0.33 | 0.784 ± 0.111 | 5.1 ± 0.3 | 1.15 ± 0.15 | 0.225 ± 0.036 |
Each value represents the mean ± S.D. of three determinations.
N.C., not calculated.