Influence of reaction conditions on the rates of (R)- and (S)-ketotifen glucuronidation in human liver microsomes
| pH | UDP-Glucuronic Acid Concentration | Triton X-100 Concentration | Substrate Concentration | Relative Reaction Rate | |
|---|---|---|---|---|---|
| (R)-Ketotifen | (S)-Ketotifen | ||||
| mM | % | μM | |||
| 7.4 | 2 | 0.02 | 50 | 0.75 | 0.84 |
| 7.7 | 2 | 0.02 | 50 | 0.92 | 0.94 |
| 8.0 | 1 | 0.02 | 5 | 0.94 | 0.80 |
| 8.0 | 1 | 0.02 | 100 | 0.93 | 0.84 |
| 8.0 | 3 | 0.02 | 5 | 0.98 | 1.05 |
| 8.0 | 3 | 0.02 | 100 | 1.09 | 1.05 |
| 8.0 | 4 | 0.02 | 5 | 1.01 | 1.11 |
| 8.0 | 4 | 0.02 | 100 | 1.02 | 1.06 |
| 8.0 | 2 | — | 5 | 0.68 | 0.56 |
| 8.0 | 2 | — | 100 | 0.42 | 0.41 |
| 8.0 | 2 | 0.01 | 5 | 0.96 | 1.03 |
| 8.0 | 2 | 0.01 | 100 | 0.86 | 0.92 |
| 8.0 | 2 | 0.03 | 5 | 0.67 | 0.69 |
| 8.0 | 2 | 0.03 | 100 | 0.70 | 0.76 |
Reaction rates are given relative to those under standard conditions (pH 8.0, 2 mM UDP-glucuronic acid, and 0.02% Triton X-100) set to 1.00.