Apparent kinetic parameters for the N-glucuronidation of (S)-ketotifen in human liver microsomes
| Parameter | HL 18 | HL 22 | HL 23 | HL 25 | Mean ± S.D. | HL 23∗ |
|---|---|---|---|---|---|---|
| K M1 | 1.3 | 1.0 | 1.7 | 1.3 | 1.3 ± 0.3 | 1.0 |
| V max1 | 70 | 63 | 76 | 31 | 60 ± 20 | 37 |
| V max1/K M1 | 54 | 63 | 44 | 24 | 46 ± 17 | 37 |
| K M2 | 69 | 54 | 160 | 84 | 92 ± 47 | 160 |
| V max2 | 480 | 470 | 520 | 300 | 440 ± 100 | 165 |
| V max2/K M2 | 6.9 | 8.6 | 3.2 | 3.5 | 5.6 ± 2.6 | 1.0 |
| r 2 | 0.9974 | 0.9994 | 0.9997 | 0.9998 | 0.9993 |
KM values are given in μM, Vmaxin pmol/min/mg protein, Vmax/KMin μl/min/mg protein.
∗, experiment in the absence of Triton X-100.