Table 1

Formation of hydroxylated testosterone metabolites in freshly isolated and cryopreserved human liver PC held in suspension at 37°C for up to 2 h

MetaboliteHydroxylated Testosterone Metabolite Formed in
Freshly isolated cellsCryopreserved cells
t = 0 mint = 30 mint = 60 mint = 120 mint = 0 mint = 30 mint = 60 mint = 120 min
pmol/min/mg protein
6β-OHT626  ± 157517  ± 155525  ± 57335  ± 52* 468  ± 124442  ± 136398  ± 71405  ± 179
2β-OHT76  ± 1862  ± 1567  ± 1139  ± 5* 62  ± 1954  ± 1451  ± 1247  ± 24

Data are expressed as mean ± S.D. of four independent human PC isolations and their corresponding cryopreserved counterparts.

  • ∗, significantly different from the corresponding t = 0 value (p < .05, Dunnett's test).