Table 3

Formation of hydroxylated testosterone metabolites in freshly isolated and cryopreserved mouse liver PC held in suspension at 37°C for up to 2 h

Metabolite	Hydroxylated Testosterone Metabolite Formed in
	Freshly isolated cells		Cryopreserved cells
	t = 0 min	t = 30 min	t = 60 min	t = 120 min	t = 0 min	t = 30 min	t = 60 min	t = 120 min
	pmol/min/mg protein
6β-OHT	136 ± 84	120 ± 87	96 ± 77	69 ± 77	137 ± 70	101 ± 60	96 ± 45	60 ± 35
15α-OHT	61 ± 47	50 ± 45	45 ± 41	41 ± 36	53 ± 35	51 ± 33	52 ± 35	46 ± 44
6α-OHT	58 ± 27	58 ± 34	54 ± 31	39 ± 27	50 ± 16	45 ± 12	46 ± 15	33 ± 17
16α-OHT	42 ± 40	38 ± 36	26 ± 20	23 ± 20	42 ± 31	29 ± 25	31 ± 23	22 ± 19
7α-OHT	42 ± 36	40 ± 39	56 ± 47	50 ± 48	48 ± 44	36 ± 33	33 ± 31	25 ± 20
2α-OHT	38 ± 23	33 ± 27	36 ± 25	30 ± 25	35 ± 7	42 ± 27	42 ± 30	34 ± 17
2β-OHT	25 ± 5	22 ± 9	17 ± 12	16 ± 6	27 ± 16	30 ± 8	40 ± 24	17 ± 11

PC from three to four mice were pooled and the results are expressed as mean ± S.D. of four such pools.