Analysis of metabolites in excreta 24 h after administration of [14C]1,2-DEB to male Sprague-Dawley rats4-a
| Treatment | Percentage of the Sample Radioactivity | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Route of Administration | Dose | Animal | Excreta | Fraction 14-a | 1,2-DEB | EPE | Fraction 24-a | GA1 | GA2 | Ratio GA1/GA2 |
| mg/kg b.wt. | ||||||||||
| i.v. | 1 | non bile duct-cannulated | urine | 9.4 ± 0.9 | <0.1 | 1.6 | 82.4 ± 1.2 | 21.6 | 40.1 | 0.5 |
| (n = 5) | feces | 27.9 ± 1.6 | <0.1 | 19.9 | 18.9 ± 0.6 | <0.1 | 1.1 | |||
| i.v. | 1 | bile duct-cannulated | urine | 7.3 ± 1.6 | <0.1 | 1.1 | 79.3 ± 4.1 | 35.5 | 43.4 | 0.8 |
| (n = 3) | bile | 4.0 ± 0.0 | <0.1 | 1.0 | 88.8 ± 0.4 | 16 | 48.5 | 0.3 | ||
| Oral | 100 | non bile duct-cannulated | urine | 6.6 ± 0.4 | <0.1 | 1.6 | 81.6 ± 1.1 | 20 | 36.9 | 0.5 |
| (n = 5) | feces | 25.5 ± 2.5 | 6.6 | 12.5 | 10.4 ± 3.4 | <0.1 | <0.1 | |||
| Oral | 100 | bile duct-cannulated | urine | 2.7 ± 0.1 | <0.1 | 0.3 | 84.0 ± 0.4 | 25.1 | 31.8 | 0.8 |
| (n = 3) | bile | 3.4 ± 0.4 | <0.1 | 1.6 | 93.9 ± 1.1 | 27.5 | 47.4 | 0.6 | ||
Urine, bile, or feces were collected on a 0- to 24-h period. For each sample, neutral (fraction 1) and acidic (fraction 2) metabolites were separated as described in Materials and Methods. The radioactivity content was measured on an aliquot of each fraction. Then, aliquots of each fraction from the same treatment were pooled and EPE, GA1, and GA2 metabolites of [14C]DEB were quantified by HPLC.
↵4-a Data expressed as mean ± S.E.