Table 2

Human serum albumin binding of chelators and iron complexes

CompoundFree LigandFe(III) Complex
Fraction unbound2-aRelative % unbound2-bFraction unbound
2 0.08271.050.343
3 0.07841.000.082
4 0.00590.070.007

  • ↵2-a Fraction of total chelator (300 μM) remaining unbound after incubation with 1 mM human serum albumin at 37°C for 30 min and ultrafiltration to remove bound ligand.

  • ↵2-b Relative percentage of unbound figures for free ligand are normalized to the value for chelator 3.