Quantitation of CYP3A immunoreactive proteins in porcine hepatocytes by Western blotting and scanning densitometry
| Microsomal Sample | Protein | Band 1 Corrected OD# | Band 2 Corrected OD | Band 3 Corrected OD | Band 4 Corrected OD |
|---|---|---|---|---|---|
| μg | OD·mm·mm | ||||
| Pig liver | 20 | 0.12 | 0.42 | 0.25 | 0.071 |
| (Control) | 40 | 0.22 | 0.52 | 0.38 | 0.20 |
| Pig liver | 10 | 0.08 | 0.76 | 0.75 | 0.20 |
| (Rifampin) | 20 | 0.12 | 1.0 | 0.96 | 0.23 |
| Control Cultures | 40 | 0.1 | 0.1 | ||
| Rifampin Cultures | 20 | 1.13 | 0.36 | ||
# Corrected for background signal and area of bands.
Blank spaces indicate bands that could not be quantified by densitometry.