Substrate | N-Oxide Reducing Activity6-anmol/30 min/mg protein | |
---|---|---|
NADH | NADH plus menadione | |
ImipramineN-oxide | 1.6 ± 0.2 | 9.8 ± 0.1 |
Cyclobenzaprine N-oxide | 1.6 ± 0.2 | 13.5 ± 1.3 |
Brucine N-oxide | 4.6 ± 0.9 | 11.4 ± 1.0 |
Nicotinamide N-oxide | 3.2 ± 0.8 | 3.1 ± 0.8 |
Each value represents the mean ± S.D. of four experiments.
↵6-a The assay was carried out using 0.2 ml of rat liver cytosol under anaerobic conditions. Reduction products formed were determined by HPLC as described in Materials and Methods.