Epoxidation of VCH in hepatic microsomes isolated from B6C3F1 mice treated with multiple doses of VCH
| Treatment | P-450 | Epoxide Metabolite | Fold Induction | ||
|---|---|---|---|---|---|
| 1,2-VCHE | 7,8-VCHE | 1,2-VCHE | 7,8-VCHE | ||
| nmol/mg | nmol/min/mg | ||||
| None | 0.52 | 4.1 ± 0.3 | 2.6 ± 0.5 | ||
| VCH | 0.87 | 11.0 ± 1.01-b | 5.9 ± 1.11-b | 2.6 | 2.2 |
Animals were treated with 7.5 mmol of VCH/kg/day i.p. for 10 days, and livers were harvested for microsomal preparation 24 h following the last dose. Microsomes (0.75 mg of protein) were incubated for 10 min with 1 mM VCH.
1-a Data represent the mean ± S.D. of triplicate incubations using three different sets of pooled microsomes.
↵1-b Statistically different from control group (p < .0001).