Epoxidation of 1,2-VCHE in hepatic microsomes isolated from B6C3F1 mice treated with different inducing agents
| Treatment | P-450 | VCD2-a | Fold Induction |
|---|---|---|---|
| nmol/mg | nmol/min/mg | ||
| None | 1.1 | 2.13 | |
| VCH | 1.6 | 4.50 | 2.1 |
| ACE | 1.5 | 4.22 | 2.0 |
| PB | 1.7 | 7.66 | 3.6 |
| DEX | 2.0 | 5.87 | 2.8 |
Animals were treated with 7.5 mmol of VCH/kg/day i.p. for 10 days or with inducing agents as described in Materials and Methods,and livers were harvested for microsomal preparation 24 h following the last dose. Microsomes (0.75 mg of protein) were incubated for 10 min with 1 mM 1,2-VCHE.
↵2-a Data represent the mean of two experiments, each performed in triplicate with pooled microsomes. Relative S.D. was <8%.