Western blot analysis (Fig. 4) using microsomes from B6C3F₁mice treated with VCH (7.5 mmol/kg/day × 10 days i.p.) or various inducing agents were prepared as described in Materials and Methods. Densitometry was performed and results have been normalized to control (i.e., nontreated mice).

• ↵4-a Values listed for CYP2B-immunoreactive protein levels do not correspond to the immunoblot in Fig. 4. The data were obtained from a blot in which 10 μg of microsomal protein was loaded (blot not shown), thus allowing for a immunoreactive band to be detected in microsomal fractions isolated from nontreated mice probed with rat CYP2B1.