Effects of enzyme inhibitors on ED-501 formation activity in rat plasma
| Inhibitor | Remaining Activity |
|---|---|
| % | |
| None | 100.0 ± 7.88 |
| 0.1mM DFP | N.D. |
| 0.1mM PMSF | 16.0 ± 0.64 |
| 0.1mM MIA | 94.0 ± 2.75 |
| 0.1mM IAA | 94.2 ± 0.96 |
| 0.1mM EDTA | 98.8 ± 2.95 |
| 1 mM EDTA | 99.6 ± 1.22 |
Rat plasma was preincubated with each inhibitor in 50 mM Tris-HC1 buffer, pH 7.4, at room temperature for 1 h, and then a residual activity was assayed. Each value represents the mean ± S.D. (n = 4). N.D. <1.4%.