Distribution of radioactivity and metabolites identified in HPLC-purified extracts of urine and feces of mice
| NOAC1-a | Ara-C | |||||||
|---|---|---|---|---|---|---|---|---|
| Urine | Feces | Urine | Feces | |||||
| Recovery in excrements (% of Dose)1-b | 39 ± 0.5 | 16 ± 2 | 48 ± 3 | 2 ± 0.5 | ||||
| HPLC1-c | Methoda | Method b | Method a | |||||
| Pool I | Pool II | Pool III | ||||||
| R t(min) | 4–5 | 5–8 | 4–6 | 8–10 | 21–23 | 4–5 | 5–8 | |
| Recovery in pools (% of Dose)1-d | 25 ± 0.3 | 11 ± 0.1 | 8 ± 0.7 | 4 ± 0.5 | 2 ± 0.1 | 33 ± 2 | 13 ± 1 | ND1-e |
| Metabolites1-f | Ara-C | Ara-U | NOAC + OSO3H | NOAC + OH | NOAC | Ara-C | Ara-U | |
↵1-a Four μmol drug per mouse.
↵1-b Accumulated over 48 h. Calculated directly from urine or feces extracts. Mean of three experiments.
↵1-c HPLC methods are described in Materials and Methods. Rt, for retention time.
↵1-d Cumulated from pools indicated in Fig. 4, A and B.
↵1-e ND, not determined.
↵1-f Detected with LC/MS in corresponding pools.