Time course of NNK and its metabolites in plasma of a rhesus monkey3-a
| Metabolite | % Distribution of Metabolites in Plasma | ||||||
|---|---|---|---|---|---|---|---|
| 5 min | 10 min | 15 min | 60 min | 90 min | 150 min | 180 min | |
| Hydroxy acid | 8.9 | 17.3 | 14.4 | 6.4 | 10.9 | 7.7 | 8.4 |
| Keto acid | 8.7 | 6.1 | 7.3 | 2.8 | 6.1 | 6.8 | 6.7 |
| NNAL-Gluc(I+II) | 7.5 | 17.1 | 14.1 | 27.3 | 25.1 | 30.5 | 33.5 |
| NNAL-N-oxide | 2.7 | 4.7 | 4.2 | 3.0 | 3.0 | N.D. | N.D. |
| Diol | N.D.3-c | 7.9 | 6.8 | 6.3 | 5.6 | 7.8 | 7.5 |
| Keto alcohol | 8.3 | 6.1 | 4.6 | 4.0 | 2.4 | 3.0 | N.D. |
| NNAL | 28.1 | 28.1 | 30.5 | 43.8 | 45.0 | 44.2 | 43.8 |
| NNK | 35.9 | 12.8 | 18.2 | 6.4 | 2.0 | N.D. | N.D. |
| Σ of α-hydroxylation3-b | 28.6 | 37.3 | 33.1 | 19.5 | 25.0 | 25.3 | 22.6 |
| NNAL + NNAL-Gluc | 35.5 | 45.2 | 44.6 | 71.1 | 70.1 | 74.1 | 77.3 |
↵3-a One female rhesus monkey (5.2 kg) received i.v. 1.72 mCi of [5-3H]NNK (28.4 μg/kg). Blood was collected for 6 h, and plasma was analyzed by HPLC as described inMaterials and Methods.
↵3-b Including hydroxy acid, keto acid, diol, and keto alcohol.
↵3-c Not detected (<1% of total radioactivity analyzed by HPLC).