Sample | ω | ω-1 |
---|---|---|
nmol/min/mg protein | ||
Liver microsomes | 1.48 ± 0.24 | 0.29 ± 0.07 |
Kidney cortical microsomes | 1.19 ± 0.01 | 0.23 ± 0.05 |
Cortical cells | 1.14 ± 0.15 | 0.21 ± 0.07 |
PT cells | 0.79 ± 0.07 | 0.20 ± 0.02 |
DT cells | 0.77 ± 0.08 | 0.18 ± 0.02 |
Activity of CYP4A was determined by analysis of the appearance of two metabolites of lauric acid (ω and ω-1) by HPLC. Results are the means ± S.E. of measurements from three separate preparations of microsomes or cells. For isolated cells, material from two animals was combined and was used for a single experiment. No significant differences (P < .05) were observed between PT and DT cells.