Chromatographic conditions for retigabine and retigabine N-glucuronide determination by HPLC
| Extraction column | Spherisorb C1, 0.5 × 4.0 mm, 5 μm (Bischoff Analysentechnik) |
|---|---|
| Washing buffer | 20 mM KH2PO4pH 7.2 |
| Flow rate; Enrichment time | 0.5 ml/min; 3 min |
| Analytical column | Spherisorb C1, 250 × 4.0 mm, 5 μm (Bischoff Analysentechnik) |
| Mobile phase | 20 mM KH2PO4 pH 7.2/Acetonitrile 77:23 (w/w) |
| Flow rate | 1.14 ml/min |
| Column temperature | Room temperature |
| Sample temperature | 8°C |
| Detection | Wavelength: 220 nm, Response time: 0.1 s, Range: 0.1 |
| Injection volume | 80–200 μl |