Addition to the Primary Reaction Mixture | Turnover Number | Activity Remaining |
---|---|---|
min−1 | % | |
None | 65 ± 1 1-a | 100 |
NADPH (1.0 mM) | 51 ± 1 | 78 |
KR-II (0.1 mM) | 59 ± 2 | 90 |
KR-II (0.1 mM) | ||
+ | 22 ± 4 | 33 |
NADPH (1.0 mM) |
The P-450 reconstituted system containing CYP2B1 and reductase was incubated for 10 min at 37°C with the compounds indicated or appropriate solvents as described in Materials and Methods.Aliquots were taken, and the activity was determined in the benzphetamine N-demethylase assay.
↵1-a Mean ± S.D., n = 3.