Concentration | Turnover Number | Activity Remaining | |
---|---|---|---|
mM | min−1 | % | |
Time 0 Control | 55 ± 1 2-a | 100 | |
Water | 17 ± 6 | 30 | |
Glutathione | 1.00 | 19 ± 2 | 34 |
Dithiothreitol | 1.00 | 14 ± 9 | 25 |
The P-450 reconstituted system containing CYP2B1 and reductase was incubated for 10 min at 37°C with NADPH (1 mM), KR-II (0.1 mM), and the compounds indicated or their solvent (water) as described inMaterials and Methods. At the end of the incubation, aliquots were taken and assayed for benzphetamineN-demethylase activity.
↵2-a Mean ± S.D., n = 3.