Comparison of NNAL- and androsterone-glucuronidating activities in liver microsomes from untreated Wistar rats
| Rat3-a | Rate of Androsterone-Gluc Formation | Androsterone-Glucuronidation Phenotype | Rate of NNAL-Gluc Formation |
|---|---|---|---|
| pmol/mg protein/min3-b | pmol/mg protein/min | ||
| 1 | 794 ± 1413-c | High3-d | 440 ± 1103-c |
| 2 | 114 ± 44 | Low | 570 ± 50 |
| 3 | 624 ± 21 | High | 490 ± 20 |
| 4 | 247 ± 33 | Low | 390 ± 30 |
| 5 | 141 ± 3 | Low | 400 ± 50 |
↵3-a Rat refers to individual rats.
↵3-b Assays performed at 37°C for 40 min using 0.5 mM androsterone and 1 mM [14C]UDPGA (0.1 μCi/reaction) as described in Materials and Methods.
↵3-c Mean ± S.D. for three experiments.
↵3-d Liver microsomes from high androsterone-glucuronidating Wistar rats exhibited an average of 4.24-fold the activity of liver microsomes from low anderosterone-glucuronidating Wistar rats.