Table 1
Mass spectrometric data from LC-ESI/MS analyses of pooled human liver microsomal incubates (20 μM, 20 min)
| Conditions1-a | Component | Ions Observed1-b1-cm/z (% relative intensity) | ||||
|---|---|---|---|---|---|---|
| [M-H]− (Containing 1 14C Atom) | M+H+ (Containing 1 14C Atom) | Fragment A (Containing 1 14C Atom) | Fragment A - H2O (Containing 1 14C Atom) | Additional Fragments with Rel. Int. ≥5% | ||
| Positive ions, 29-V collision offset | CA | 324 (100)1-d | 143 (92)1-d | |||
| D-DHD1 | 314 (100)1-d | 177 (16) | 159 (17) | |||
| D-DHD2 | 314 (100)1-d | |||||
| DOL | 296 (88)1-d | 143 (100)1-d | 119 (11) | |||
| DHD1 | 328 (100)1-d | 177 (8)1-d | 159 (9)1-d | |||
| DHD2 | 328 (100)1-d | 159 (3) | ||||
| OL | 310 (94)1-d | 143 (100)1-d | 119 (10) | |||
| D | 280 (47)1-d | 143 (100)1-d | 121 (6), 93 (7) | |||
| terbinafine | 294 (39)1-d | 143 (100)1-d | 93 (7) | |||
| Negative ions, 10-V collision offset | NA | 173 (100) | ||||
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↵1-a Offset voltage applied in the ion source region (between skimmer and transfer-octopole) for declustering and collision-induced fragmentation.
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↵1-b Parent drug 88% [14C]-labeled; ions containing the label appearing two mass units higher than without label.
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↵1-c Fragment A: cleavage of benzylic type C-N bond with charge retention on the aromatic ring system.
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↵1-d Accompanying minor signal of the all-12C isotopomer observed two mass units lower.