Summary of assay conditions for fluoxetine N-demethylation in heterologously expressed recombinant human CYP enzymes
| Enzyme | Microsomal Protein Concentration | P450 Concentration | Fluoxetine Concentration Range | Incubation Time |
|---|---|---|---|---|
| mg/ml | pmol/ml | μM | min | |
| CYP1A2 | 0.3 | 12 | 5.0–500 | 20 |
| CYP2B6 | 0.4 | 53 | 5.0–500 | 20 |
| CYP2C9 | 0.15 | 7.1 | 1.0–100 | 15 |
| CYP2C19 | 0.6 | 19 | 10–1000 | 5 |
| CYP2D6 | 0.088 | 16 | 0.25–25 | 15 |
| CYP3A4 | 0.6 | 29 | 5.0–500 | 5 |
| CYP3A5 | 1.0 | 10 | 5.0–500 | 15 |