Homogenate | Km | S.E. | Vmax | S.E. | Ki | χ2 | Cli |
---|---|---|---|---|---|---|---|
μM | pmol/min/mg | μM | μl/min/mg | ||||
Liver donor no. 1 | 1547 | 55.8 | 89.0 | 1.23 | 9.1 | 1.91 | 0.058 |
Liver donor no. 2 | 1707 | 64.6 | 131 | 1.86 | 0.077 | ||
Liver donor no. 3 | 1211 | 40.8 | 60.8 | 0.68 | 6.8 | 2.12 | 0.050 |
Small intestine donor no. 2 | 2149 | 311 | 11.1 | 0.51 | 0.005 | ||
Small intestine donor no. 3 | 1565 | 219 | 5.80 | 0.28 | 0.004 |
Mean Km and Vmax of codeine-6-glucuronidation in liver homogenate: 1488 μM, 93.6 pmol/min/mg; mean Km andVmax of codeine-6-glucuronidation in enterocyte homogenate (small intestine): 1857 μM, 8.5 pmol/min/mg; meanKi of diclofenac on codeine-6-glucuronidation in liver homogenate: 7.9 μM.