Table 4

Kinetic parameters of acenocoumarol and warfarin hydroxylations in human liver microsomes (sample HD10)

Substrate	Metabolite	K_m	V_max	V_max/K_m
		μM	pmol · min⁻¹ · mg	μl · min⁻¹ · mg
(R)-AC^4-a	6-OH 1	30.8	16.8	0.54
	6-OH 2^4-b	117	37.0	0.32
	7-OH	16.9	8.4	0.49
	8-OH 1	14	4.1	0.32
	8-OH 2^4-b	50	7.3	0.15
(S)-AC	6-OH	0.47	10.4	21.2
	7-OH	0.35	12.6	36.0
	8-OH	0.60	1.2	2.0
(R)-W	6-OH 1	64.0	4.8	0.07
	6-OH 2^4-b	380	22.6	0.06
	7-OH	85.4	5.3	0.06
(S)-W	6-OH 1	2.5	0.79	0.32
	6-OH 2^4-b	196	7.2	0.04
	7-OH	2.1	1.8	0.86

The data are the mean of incubations in triplicate.